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The Journal of Immunology, 1980, 124: 173-177.
Copyright © 1980 by The American Association of Immunologists, Inc.
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The Journal of Immunology, Vol 124, Issue 1 173-177, Copyright © 1980 by American Association of Immunologists

ARTICLES

A new lymphocyte surface protein present in normal urine. I. Isolation and physicochemical properties

NJ Calvanico, JA Velosa, WL Anderson and TB Tomasi Jr

A lymphocyte surface glycoprotein designated urinary acidic antigen (UA) has been isolated from normal urine by a combination of preparative isoelectric focusing and ammonium sulfate precipitation. It has an m.w. of 14,000 to 17,500 daltons, and is approximately 60% carbohydrate and 40% amino acid in content. The protein exhibited the following physical properties: S20,omega = 1.24; v = 0.693 ml/g; E1%1 cm, 278 nm = 2.08; and pI-2.5. It appears to be unrelated to beta 2- microglobulin, protein HC, urinary proteose, microglobulin, or any previously described normal urine or human serum protein.




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