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The Journal of Immunology, 1979, 123: 2261-2264.
Copyright © 1979 by The American Association of Immunologists, Inc.
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Isolation of the Fourth Component (C4) of Rat Complement

Mohamed R. Daha1 and Leendert A. van Es

From the Department of Nephrology, University Hospital, Leiden, The Netherlands

Abstract

The fourth component of rat complement was purified to homogeneity by sequential chromatography of rat plasma in benzamidine on QAE-A50, SP-C50, hydroxyapatite, and gel filtration on Bio-Gel A 1.5. The final material was homogeneous on SDS-PAGE analysis and had a calculated m.w. of 198,000. A monospecific antibody against rat C4 was obtained from immunized rabbits. The concentration of rat C4 in the plasma of normal 4-month-old Wistar rats was 190 ± 34 µg/ml (mean ± 1 S.D.).

Footnotes

1 Correspondence to: Dr. M. R. Daha, Department of Nephrology, University Hospital, 2333 AA Leiden, The Netherlands.






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