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The Journal of Immunology, 1979, 123: 2231-2238.
Copyright © 1979 by The American Association of Immunologists, Inc.
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Rabbit B Spleen Lymphocytes and T Helper Cells

I. Responsiveness to Mitogens of B Cell Subpopulations of Different Sedimentation Velocities and Subpopulations Bearing or Lacking Fc{gamma} Receptors1

J. M. Cavaillon2, T. N. S. Udupa3, C-T. Chou, B. Cinader4, N. Haeffner-Cavaillon5 and S. Dubiski

From the Institute of Immunology, Departments of Medical Genetics, Clinical Biochemistry and Medical Biophysics, University of Toronto, Toronto, Canada

Abstract

The response to anti-allotype (anti-Ab4), Nocardia Water Soluble Mitogen (NWSM), pneumococcal polysaccharide type III (SSS III), and human Fc fragments of various purified and unfractionated rabbit spleen cell populations was determined in terms of 3H-thymidine uptake. B cells were isolated either from untreated suspensions of spleen cells or from suspensions from which adherent and phagocytic cells were removed. The purification factor was greater than the enhancement of 3H-thymidine uptake by anti-Ab4, NWSM, and SSS III as compared with the response of unfractionated spleen cells. It thus appears that a helper cell was involved: the mitogen response of purified B cells was enhanced by the addition of T cells. B subpopulations were separated by sedimentation or by rosetting, which allowed us to separate Fc{gamma} receptor-bearing cells from cells that did not possess this receptor. There were differences between cells responding to B mitogens not only in sedimentation velocity but also in the absolute number of cells. B cells bearing the Fc{gamma} receptor were less responsive to anti-Ab4 and more responsive to SSS III, NWSM, and human Fc than were B cells lacking the Fc{gamma} receptor.

Footnotes

1 This work was supported by grants from the Medical Research Council and the National Cancer Institute of Canada.

2 Personal support from the Medical Research Council. Present address: Unite des Antigenes Bacteriens, Institut Pasteur, 28 rue du Dr. Roux, 75015, Paris, France.

3 Recipient of research training grant from the World Health Organization. Present address: Institute for Research in Reproduction, Jehangir Merwanji Street, Parel, Bombay 400 012, India.

4 Correspondence: Dr. B. Cinader, Institute of Immunology, Medical Sciences Building, University of Toronto, Toronto, Ontario, M5S 1A8, Canada.

5 Fellow of the National Research Council of Canada.






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