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Specific Stimulation and Suppression of a Primary in Vitro Plaque-Forming Cell Response by Monovalent Lipid Haptens in Fluid Liposomal Membranes¹

From the Stauffer Laboratory for Physical Chemistry, Stanford University, Stanford, California 94305

Abstract

Multilamellar, "fluid" liposomes containing an appropriate concentration of a synthetic dinitrophenylated lipid hapten behave as T-independent (TI), adherent cell-dependent antigens for primary, in vitro, stimulation of hapten-specific IgM plaque-forming cells (PFC). The appropriate concentration of hapten is approximately 1 mol% with respect to the other liposomal lipids, cholesterol, and dimyristoylphosphatidylcholine (DMPC). At a higher hapten concentration, 4 mol%, liposomes will suppress the anti-dinitrophenyl PFC response to co-cultured immunogenic liposomes, but not the anti-sheep cell response to co-cultured sheep cells. This suppression is also TI. The ability of "fluid," haptenated liposomes specifically to stimulate or to suppress the TI PFC response is determined by the ratio of hapten to other lipids in a manner that is independent of the total hapten concentration in culture.

Footnotes

¹ This work was supported by National Institutes of Health Research Grant 1R23 AI14813-01 and also, in part, by National Institutes of Health Research Grant 5R01 AI13587-03 (PI Harden M. McConnell).