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Surface Ig Isotypes on Cells Responding to Lipopolysaccharide by IgM and IgG Secretion¹

From Division of Immunology, Department of Medicine and Department of Pathology, Stanford University, School of Medicine, Stanford, California 94305, and Department of Microbiology, University of Texas, Southwestern Medical School, Dallas, Texas 75235

Abstract

Using the fluorescence-activated cell sorter (FACS), we have investigated the Ig isotypes present on murine B cells, which can be polyclonally activated by lipopolysaccharide (LPS) in low cell density cultures. The LPS response was partly inhibited as a result of staining with anti-IgD and anti-IgM reagents, but not with anti-IgG reagents. The IgM⁺, IgD⁺, or IgG^- fractionated cell populations gave both an IgM and an IgG response comparable to controls, whereas the response of the IgM^-, IgD^- cells was 5- to 20-fold lower. IgG^- cells separated 1 day after LPS stimulation could still mount an IgM and IgG response indistinguishable from controls at the peak of the response. It is concluded that IgM⁺, IgD⁺, IgG^- cells constitute the major LPS-sensitive cell population in the low cell density culture system and that IgG is not a necessary cell surface isotype for precursors of IgG-secreting cells.

Footnotes

¹ This work was supported by National Institutes of Health Grants AI 10293, 11851, and 12789.

² Recipient of a senior fellowship from the American Cancer Society, California Division. Present address: Division of Immunobiology, Wallenberg Laboratory, Lilla Freskati, S-10405 Stockholm, Sweden. To whom correspondence should be sent.

³ Recipient of a fellowship from the Leukemia Society of America.

⁴ Investigator, Howard Hughes Medical Institute.

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