HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Santoro, F. Articles by Capron, M. Search for Related Content PubMed Articles by Santoro, F. Articles by Capron, M.

Activation of Complement by Schistosoma Mansoni Schistosomula: Killing of Parasites by the Alternative Pathway and Requirement of IgG for Classical Pathway Activation¹

Centre d'Immunologie et de Biologie Parasitaire (INSERM U167), Institut Pasteur de Lille (France), and MRC Group on Mechanisms in Tumour Immunity, The Medical School, Cambridge (U. K.)

Abstract

Living Schistosoma mansoni schistosomula incubated with normal chicken, guinea pig, human, and monkey sera were killed after 4 hr contact at 37°C. The following data indicate that this action is dependent on the activation of the alternative complement pathway (AP): a) the inactivity of RB, RD, and zymosan-treated serum against schistosomula; b) the partial activity of RD restored in FD; c) the full effect of the C4-deficient guinea pig, C2-deficient human, and the agammaglobulinemic human sera; d) the consumption of both the AP and FB after the incubation of NHS with schistosomula; e) the detection of C3d breakdown product during the contact of the C2-deficient human serum with these young parasites.

Killing by serum was decreased as the immature schistosomes developed and was completely absent against 4-day-old lung schistosomula (LS). In other experiments, it was demonstrated that schistosomula, in the presence of IgG, were able to initiate complement activation also through the classical pathway (CP). However, the CP does not appear to play a role in the schistosomulicidal activity of complement. The in vivo relevance of these observations is considered.

Footnotes

¹ This work was supported by grants from the INSERM (U 167), CNRS (ERA 422), and WHO Special Program for Research and Training in Tropical Diseases.

² Address correspondence to Dr. Ferrucio Santoro, Centre d'Immunologie et de Biologie Parasitaire, Institut Pasteur, B.P. 3415, 59019-Lille Cedex, France.

This article has been cited by other articles:

				P. R. Giacomin, H. Wang, D. L. Gordon, M. Botto, and L. A. Dent Loss of Complement Activation and Leukocyte Adherence as Nippostrongylus brasiliensis Develops within the Murine Host Infect. Immun., November 1, 2005; 73(11): 7442 - 7449. [Abstract] [Full Text] [PDF]