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Cellular Immunobiology Unit, University of Alabama in Birmingham, Alabama 35294, and the Institute for Genetics, University of Cologne, Weyertal 121, D-500 Köln 41, F. R. G.
Abstract
We have isolated a subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains. This clone X63-Ag8.653 can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies. Screening of hybrid cell lines for specificity and immunoglobulin classes was done with a modified enzyme-linked immunosorbent assay.
Footnotes
1 This work was supported by the Deutsche Forschungsgemeinschaft through Sonderforschungsbereich 74 and Grants CA 16673 and A 114782. J. K. was supported by an EMBO senior fellowship and is a recipient of RCDA award 1 K04 AI/CA 00338.
2 Address correspondence to Dr. J. Kearney, 224 Tumor Institute, University Station, University of Alabama in Birmingham, Birmingham, Alabama 35294.
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 W Halfter, B Schurer, H. Hasselhorn, B Christ, E Gimpel, and H. Epperlein An ovomucin-like protein on the surface of migrating primordial germ cells of the chick and rat Development, January 3, 1996; 122(3): 915 - 923. [Abstract] [PDF]
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 R. Allen, N. Bala, R. Ali, D. Nishida, M. Aihara, M Ishida, and A. Fok Rapid bulk replacement of acceptor membrane by donor membrane during phagosome to phagoacidosome transformation in Paramecium J. Cell Sci., January 3, 1995; 108(3): 1263 - 1274. [Abstract] [PDF]
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R Pasqualini, J Bodorova, S Ye, and M. Hemler A study of the structure, function and distribution of beta 5 integrins using novel anti-beta 5 monoclonal antibodies J. Cell Sci., January 5, 1993; 105(1): 101 - 111. [Abstract] [PDF]
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 B. Chan, N Matsuura, Y Takada, B. Zetter, and M. Hemler In vitro and in vivo consequences of VLA-2 expression on rhabdomyosarcoma cells Science, March 29, 1991; 251(5001): 1600 - 1602. [Abstract] [PDF]
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 D. Deutsch, A. Palmon, J. Catalano-Sherman, and R. Laskov Production of Monoclonal Antibodies Against Enamelin and Against Amelogenin Proteins of Developing Enamel Matrix Advances in Dental Research, December 1, 1987; 1(2): 282 - 288. [Abstract] [PDF]
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G Kohler Derivation and diversification of monoclonal antibodies Science, September 19, 1986; 233(4770): 1281 - 1286. [PDF]
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D Herlyn, A. Ross, and H Koprowski Anti-idiotypic antibodies bear the internal image of a human tumor antigen Science, April 4, 1986; 232(4746): 100 - 102. [Abstract] [PDF]
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R. Perlmutter, J. Kearney, S. Chang, and L. Hood Developmentally controlled expression of immunoglobulin VH genes Science, March 29, 1985; 227(4694): 1597 - 1601. [Abstract] [PDF]
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 W.B. Stallcup, L. Beasley, and J. Levine Cell-surface Molecules That Characterize Different Stages in the Development of Cerebellar Interneurons Cold Spring Harb Symp Quant Biol, January 1, 1983; 48(0): 761 - 774. [Abstract] [PDF]
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C.J. Barnstable, K. Akagawa, R. Hofstein, and J.P. Horn Monoclonal Antibodies That Label Discrete Cell Types in the Mammalian Nervous System Cold Spring Harb Symp Quant Biol, January 1, 1983; 48(0): 863 - 876. [Abstract] [PDF]
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 R. A. Insel and F. Gigliotti Monoclonal Antibodies: Clinical Relevance to Pediatrics Arch Pediatr Adolesc Med, January 1, 1983; 137(1): 69 - 76. [Abstract] [PDF]
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R. P. Perry, C. Coleclough, and M. Weigert Reorganization and Expression of Immunoglobulin Genes: Status of Allelic Elements Cold Spring Harb Symp Quant Biol, January 1, 1981; 45(0): 925 - 933. [Abstract] [PDF]
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