The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

The Journal of Immunology, 1979, 123: 1504-1510.
Copyright © 1979 by The American Association of Immunologists, Inc.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kishimoto, T.
Right arrow Articles by Yamamura, Y.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Kishimoto, T.
Right arrow Articles by Yamamura, Y.

Involvement of Anti-Ig-Activated Serine Protease in the Generation of Cytoplasmic Factor(s) that Are Responsible for the Transmission of Ig-Receptor-Mediated Signals1

Tadamitsu Kishimoto, Hitoshi Kikutani, Yoshio Nishizawa, Nobuo Sakaguchi and Yuichi Yamamura

Third Department of Internal Medicine, Osaka University Medical School, Fukushima-ku, Osaka, Japan

Abstract

Involvement of serine protease-activation in the generation of cytoplasmic factor(s) that induced NHP-specific protein kinase activity in nuclei in anti-Ig-stimulated cells was described. DFP or PMSF with anti-Ig inhibited the induction of cytoplasmic factor(s), whereas pretreatment of cells with DFP or PMSF without anti-Ig did not show any inhibitory effect on anti-Ig-induced generation of cytoplasmic factor(s). TAME or BAME with anti-Ig inhibited the generation of cytoplasmic factor(s) and the simultaneous addition of TAME or BAME with DFP protected the generation of cytoplasmic factor(s) against the inhibitory effect of DFP, showing the involvement of trypsin-like, arginine-type serine protease in anti-Ig-induced generation of cytoplasmic factor(s). Anti-Ig-stimulated membrane preparations induced cytoplasmic factor(s) in normal cytoplasm. The m.w. of precursor proteins present in resting B cells and active cytoplasmic factor(s) were approximately 150,000 and 45,000, respectively. These results showed that anti-Ig-activated membrane-bound serine protease split precursor proteins in resting B cells into active cytoplasmic factor(s) responsible for signal transmission.

Footnotes

1 This work was supported by a Grant-in-Aid for immunological researches from the Ministry of Education, Science and Culture, Japan.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1979 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1979 by The American Association of Immunologists, Inc. All rights reserved.