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The Journal of Immunology, 1979, 123: 1299-1302.
Copyright © 1979 by The American Association of Immunologists, Inc.
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Nature of T Lymphocyte Recognition of Macrophage-Associated Antigens

II. Macrophage Determination of Guinea Pig T Cell Responses to Human Fibrinopeptide B1

David W. Thomas, Susan K. Meltz and George D. Wilner

Department of Pathology and Laboratory Medicine, The Jewish Hospital of St. Louis, and the Departments of Pathology and Microbiology-Immunology, Washington University School of Medicine, St. Louis, Missouri 63110

Abstract

Strain 2, strain 13, or (2 x 13)F1 guinea pigs were immunized with human fibrinopeptide B, a 14 amino acid thrombin-derived fragment of the Bbeta chain of fibrinogen (Bbeta1-14), or the Bbeta1-13 homologue, and in vitro T lymphocyte responsiveness was determined by the incorporation of 3H-thymidine. T cells from strain 2 or (2 x 13)F1 animals immunized with Bbeta1-14 showed proliferative responses with Bbeta1-14 but were unresponsive with Bbeta1-13. Bbeta1-13, Strain 13 Bbeta1-14 immune T cells were unresponsive with both Bbeta1-14 and 1–13. By contrast, T cells from strain 13 or (2 x 13)F1 guinea pigs immunized with Bbeta1-13 produced proliferative responses with Bbeta1-13 but not with Bbeta1-14. Strain 2 Bbeta1-13 immune T cells showed minimal or no responsiveness with Bbeta1-13 or 1–14. Thus, responsiveness to Bbeta1-14 is associated with strain 2 animals, and responsiveness to Bbeta1-13 is associated with strain 13 guinea pigs. To determine the role of macrophages in presentation of Bbeta1-14 or 1–13, T cells from (2 x 13)F1 guinea pigs immunized with Bbeta1-14 or 1–13 were stimulated by antigen in association with macrophages derived from either parent. F1 T cells immunized with Bbeta1-14 responded to Bbeta1-14 in association with only strain 2 but not strain 13 macrophages. By contrast, Bbeta1-13 immune F1 T cells responded with Bbeta1-13 in association with only strain 13 but not strain 2 macrophages. These results are discussed with respect to the role of macrophages in selecting immunogenic determinants of peptide antigens for presentation to T cells.

Footnotes

1 This work was supported by United States Public Health Service Grant AI-14226 from the National Institute of Allergy and Infectious Diseases, Grants HL-15486 and HL-22642 from the National Heart and Lung Institute, National Institutes of Health; and Biomedical Research Support Grant RR-0549 to The Jewish Hospital of St. Louis from the Division of Research Resources.






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