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Potentiation of Antibody-Dependent Cell-Mediated Cytotoxicity by Target Cell-Bound C3b¹

Department of Molecular Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037

Abstract

Antibody-dependent cytolytic effector lymphocytes are known to possess, in part, receptors for activated C3. Employing a model system consisting of ⁵¹Cr-labeled chicken erythrocytes and purified human peripheral lymphocytes, we investigated the effect of target cell bound C3b on antibody-dependent cellular cytotoxicity (ADCC). At concentrations of anti-target cell antibody too low to cause effective ADCC, target cell bound C3b cooperated with antibody to produce marked target cell lysis. In the presence of a 1/6.25 x 10⁶ dilution of antichicken erythrocyte rabbit IgG, cell lysis increased from 20% to 65% by the attachment of 18,000 C3b molecules per cell. C3b-dependent enhancement of ADCC was dose dependent. It was augmented by attachment of activated properdin () to the C3b-bearing target cells.

Footnotes

¹ This is Publication Number 1752 from the Research Institute of Scripps Clinic. This investigation was supported by United States Public Health Service Grants AI 07007 and HL 16411.

² Present address: Université de Lausanne, Institut de Biochimie, Chemin des Boveresses, CH-1066 Epalinges sur Lausanne, Switzerland.

³ Cecil H. and Ida M. Green Investigator in Medical Research, Research Institute of Scripps Clinic.