HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Finkelman, F. D. Articles by Scher, I. Search for Related Content PubMed Articles by Finkelman, F. D. Articles by Scher, I.

Demonstration of Mouse Serum IgD¹

Department of Medicine, Uniformed Services University of the Health Sciences, the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, and the Department of Immunology, Naval Medical Research Institute, Bethesda, Maryland 20014

Abstract

Although the mouse would provide an excellent experimental model for the study of serum IgD function, previous investigations have failed to detect serum IgD in this species. We have developed an immunofluorescence inhibition assay that has allowed us to identify and quantitate mouse serum IgD. Mouse sera or serum fractions were assayed for their abilities to inhibit staining of mouse spleen cells with an allotype-specific fluorescein isothiocyanate-labeled rabbit antibody against mouse cell-surface IgD (FITC-RaM^a), as analyzed with a fluorescence-activated cell sorter. FITC-RaM^a stains surface IgD⁺ spleen cells of mouse strains that have surface(s) sIgD of the a allotype, such as BALB/c, but not strains that have surface IgD of the b allotype, such as C57BL/6 or C.B20, which have the C57BL/6 C_H genome on a BALB/c background. The staining of BALB/c lymphocytes by FITC-RaM^a was inhibited by BALB/c serum, but not by C57BL/6 or C.B20 serum. The staining was also not inhibited by BALB/c sera that had been adsorbed with rabbit anti-mouse Ig or a mouse hybridoma protein anti-mouse , or by BALB/c IgG, IgM, or IgA plasmacytoma proteins. These data strongly suggest that mice have IgD in their serum. Mouse serum IgD is probably secreted rather than shed from B cells, since amounts of serum IgD and splenic IgD in the same mice did not correlate with each other and serum IgD and shed sIgD had different density profiles.

Footnotes

¹ Supported in part by the Uniformed Services University of the Health Sciences Research Nos. R08307, R08308, and C08310, and in part by the Naval Medical Research and Development Command, Work Unit No. M0095-PN.001-1030. The opinions and assertions contained herein are the private ones of the writers and are not to be construed as official or reflecting the views of the Navy Department or the naval service at large. The experiments reported herein were conducted according to the principles set forth in the "Guide for the Care and Use of Laboratory Animals," Institute of Laboratory Resources, National Research Council, Department of Health, Education and Welfare, Pub. No. (NIH) 78-23.

This article has been cited by other articles:

				I. Levan-Petit, E. Lelievre, A. Barra, A. Limosin, B. Gombert, J.-L. Preud'homme, and J.-C. Lecron Th2 cytokine dependence of IgD production by normal human B cells Int. Immunol., November 1, 1999; 11(11): 1819 - 1828. [Abstract] [Full Text] [PDF]

				M. White, A. Shen, C. Word, P. Tucker, and F. Blattner Human immunoglobulin D: genomic sequence of the delta heavy chain Science, May 10, 1985; 228(4700): 733 - 737. [Abstract] [PDF]

				C. Liu, P. Tucker, J. Mushinski, and F. Blattner Mapping of heavy chain genes for mouse immunoglobulins M and D Science, September 19, 1980; 209(4463): 1348 - 1353. [Abstract] [PDF]

				P. Tucker, C. Liu, J. Mushinski, and F. Blattner Mouse immunoglobulin D: messenger RNA and genomic DNA sequences Science, September 19, 1980; 209(4463): 1353 - 1360. [Abstract] [PDF]