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In Vitro Immune Response of Human Peripheral Lymphocytes

IV. Specific Induction of Human Suppressor T Cells by an Antiserum to the T Leukemia Cell Line HSB¹

Third Department of Internal Medicine, Osaka University Medical School, Fukushima-ku, Osaka, 553, Japan, and the Memorial Sloan-Kettering Cancer Center, New York, New York 10021

Abstract

An antiserum to a human T leukemia cell line, HSB, absorbed with the homologous B cell line, SB, reacted with three of five T cell lines, none of five B cell lines, and none of three other human hematopoietic cell lines. The absorbed antiserum (HSB) killed 23 to 56% normal peripheral blood lymphocyte (PBL) T cells, depending on the donor, but not B cells. Treatment of purified PBL T cells with HSB and complement (C) had no specific effect on their mitogenic responses of PHA and Con A mitogens, but partially inhibited MLC reactions. HSB and C had no effect on helper T cells or B lymphocytes in pokeweed mitogen (PWM)-induced immunoglobulin (Ig) production. When continuously present in the culture, HSB at 1:400 dilution almost completely inhibited IgG and IgM production induced by PWM. T cells pretreated with HSB for 3 days and washed were capable of inhibiting Ig production by normal PBL at ratios less than 1:100. In contrast, HSB-treated B cells had no effect on PWM induction of Ig. The suppressor T cells induced by HSB carried an antigen recognized by the antiserum on their surface as shown by removal of suppression by pretreatment with HSB and C. HSB-treated T cells secreted a soluble factor suppressing Ig production by PWM-stimulated PBL.

Footnotes

¹ This work was supported by Grants CA-08748, CA-17404, CA-24300, and NS-11457 from the National Institutes of Health; PCM75-19734 from the National Science Foundation; United States-Japan Cooperative Science Program supported by the National Science Foundation and JSPS; Special Projects Grant from MSKCC; and by grants from the Ministry of Education, Science and Culture and from the Ministry of Health and Welfare, Japan.

² All correspondence should be addressed to T. K.

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