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Lymphocytes Surface Membrane Immunoglobulin in Myeloma

I. M315-Bearing T Lymphocytes in Mice with MOPC-315¹

Department of Pathology and Division of Biology and Biomedical Sciences, Washington University School of Medicine, St. Louis, Missouri 63110

Abstract

MOPC-315 is a BALB/c plasmacytoma that produces an IgA anti-TNP antibody (M315). 315/+ is a line derived from MOPC-315 and differentiates during in vivo growth from M315-producing, nonsecreting, lymphocytoid cells to large M315-secreting plasmacytoid cells. Studies were conducted to determine whether the small M315-bearing mononuclear cells, which account for more than one-third of the circulating mononuclear cells in mice with 315/+ tumors, were myeloma cells or host lymphocytes. Experiments conducted in CBF₁ mice demonstrated that the circulating M315-bearing cells were of F₁, rather than tumor, origin. Serologic and ultrastructural studies demonstrated that the cells were post-thymic T lymphocytes. After proteolytic removal, surface M315 was reexpressed in vitro by 315/+ cells, but not by T cells. M315-bearing T cells accounted for 0 to 4% of circulating mononuclear cells in mice with 315/P, a variant of MOPC-315 in which all cells synthesize M315, but only 2% of the cells are secretory. There was no obvious relationship between the frequency of M315-bearing lymphocytes and development of the humoral immunodeficiency that accompanies myeloma. These findings 1) identify an association between high levels of M315 secretion and development of M315-bearing T cells; 2) favor the view that M315 is acquired, rather than produced by host T cells; 3) raise the possibility that T cells with IgA-Fc receptors may be increased in mice with 315/+ tumors; and 4) are discussed relative to the mechanisms that have been proposed in the literature to account for the occurrence and significance of circulating, M-component-bearing lymphoid cells in murine and human myeloma.

Footnotes

¹ This work was supported by United States Public Health Service research Grants CA17114 and CA23217 and by a special grant to the Department of Pathology from the following companies: Brown & Williamson Tobacco Corporation; Larus and Brother Company, Inc.; Liggett & Meyers, Incorporated; Lorillard, a Division of Loews Theatres, Incorporated; Philip Morris, Incorporated; R. J. Reynolds Tobacco Company; United States Tobacco Company; and Tobacco Associates, Inc.

² Supported by United States Public Health Service Training Grant GM-09118.

³ Supported by Medical Scientists Training Program and Grant GM-02016.

⁴ Reprint requests: 660 South Euclid Avenue, St. Louis, Missouri 63110.