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Characterization of a Spontaneous Murine B Cell Leukemia (BCL₁)

II. Tumor Cell Proliferation and IgM Secretion After Stimulation by LPS¹

Howard Hughes Medical Institute Laboratory-Stanford University Medical Center and the Division of Immunology, Department of Medicine, Stanford University, Stanford, California 94305, and the Folkhälsan Institute of Genetics, Helsinki, Finland

Abstract

A spontaneous BALB/c B lymphocyte leukemia could be stimulated in vitro by the polyclonal B cell activator lipopolysaccharide (LPS) and the conditions for activation were studied. Spleen cells or peripheral blood lymphocytes from tumor-bearing animals responded by increased DNA synthesis and the peak of activation occurred earlier than with normal mouse spleen cells. Tumor cells harvested from the spleen, but not from the peripheral blood, could be induced by LPS to secrete IgM. Direct demonstration that the response was due to tumor cell activation and not that of contaminating normal B lymphocytes was provided by karyotype analysis and by immunoprecipitation, which showed the restriction of light chains on secreted IgM molecules to the isotype.

Footnotes

¹ This work was supported by National Institutes of Health Grant AI 10293.

² Senior Fellow, American Cancer Society, California Division.

³ Investigator, Howard Hughes Medical Institute.

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