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The Relationship Between Surface Immunoglobulin Isotype and Immune Function of Murine B Lymphocytes

IV. Role of IgD-Bearing Cells in the Propagation of Immunologic Memory¹

From the Howard Hughes Medical Institute Laboratory, Stanford University Medical Center, and Division of Immunology, Department of Medicine, Stanford University School of Medicine, Stanford, California 94305 and the Department of Microbiology, University of Texas, Southwestern Medical School, Dallas, Texas 75235

Abstract

We developed a quantitative procedure to examine the ability of memory B cells to give rise to AFC, and to more memory B cells (propagation of memory) after antigenic stimulation. Sublethally irradiated (BALB/c x C57BL/Ka)F₁ mice were given an excess of BSA-primed T cells, graded numbers of DNP-primed B cells, and challenged with DNP-BSA in saline. The anti-DNP response of these adoptive (intermediate) hosts measures the production of AFC. After a rest period of 8 to 12 weeks, spleen cells from these intermediate hosts were transferred to sublethally irradiated final hosts. The latter animals were also given an excess of primed T cells and challenged with DNP-BSA. The anti-DNP response of the final hosts measures the ability of memory B cells to propagate memory.

The relationship between the expression of surface immunoglobulin isotype (IgM, IgD, IgG), and the ability to produce AFC or propagate memory was studied by purifying populations of IgM⁺, IgD⁺, and IgG⁺ memory B cells on the FACS. The experimental results show that IgM⁺, IgD⁺, and IgG⁺ cells give rise to AFC, but that only the IgD⁺ subset can propagate memory. This suggests that memory B cells lacking surface IgD undergo terminal differentiation after antigenic stimulation, but that those bearing IgD are capable of self-renewal.

Footnotes

¹ This work was supported by National Institutes of Health Grants AI 10293, 11851, and 12789.

² Postdoctoral Fellow of the Arthritis Foundation. Present address: Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.

³ Investigator, Howard Hughes Medical Institute.

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