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IgD Is Present on the Cell Surface of Murine Lymphocytes in Two Forms: ₂L₂ and L¹

From the Department of Microbiology, The University of Texas Health Science Center at Dallas, Dallas, Texas 75235

Abstract

Murine cell surface immunoglobulin (Ig) isotypes were analyzed by lactoperoxidase-catalyzed radioiodination, immunoprecipitation, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE analysis revealed three radioactive peaks: I) IgM (µ₂L₂; 224,000 daltons), II) IgD (₂L₂; 185,000 daltons), and III) IgD half-molecules (L; 105,000 daltons). The size of the -chain obtained from both the ₂L₂ and the L molecules was the same (68,000 daltons). The L molecules are apparently not the result of a sulfhydryl-catalyzed reduction or disulfide interchange and are probably not generated by proteolysis, since addition of alkylating agents and protease inhibitors did not decrease the amount of L observed. Addition of sulfhydryl-oxidizing agents also did not decrease the amount of L, suggesting that the L molecules cannot be reoxidized to yield ₂L₂ molecules. The percentage of IgD in the L form present on lymph node and spleen cells of A/J mice and on spleen cells of BALB/c mice was the same (55%). The percentage of L on splenocytes from BDF/1 mice (2 to 7 months old) was 42%. Spleen cells from strains that are "IgD-deficient," CBA/N and NZB, and spleen cells from 2-week-old BDF/1 mice, which have a low /µ ratio, displayed a coordinate decrease in the levels of both ₂L₂ and L. This coordinate decrease resulted in no alternation in the percentage of IgD present in the L form (42%). These results, taken together, indicate that indeed the cell surface Ig of murine lymphoid cell populations consists of monomeric IgM, monomeric IgD, and IgD half-molecules.

Footnotes

¹ This work was supported by United States Public Health Service Grant AI 11851.

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