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Cloning of B Cells Positive or Negative for Surface IgD

I. Triggering and Tolerance in T-Independent Systems¹

From the Walter and Eliza Hall Institute of Medical Research, Post Office, Royal Melbourne Hospital, Victoria 3050, Australia

Abstract

A direct experimental approach has been used to test two hypotheses for the function of B lymphocyte surface IgD (sIgD). The first hypothesis is that the presence of sIgD distinguishes cells that respond to T-dependent antigens from those that respond to T-independent antigens and the second is that the presence of sIgD is responsible for resistance to tolerance induction. The approach used was to separate sIgD⁺ cells from sIgD^- cells in the fluorescence-activated cell sorter and to use cloning assays in which the precursor activity is linearly related to the number of cells assayed, to test the activity of separated populations. Mice at 2 weeks of age were used in most experiments to provide spleen cells in which there was an equal proportion of sIgD⁺ and sIgD^- B cells. In this paper, the immune response and tolerance induction were examined in T-independent assay systems. Both sIgD⁺ and sIgD^- cells responded to a T-independent antigen (FLU-POL) and mitogens, with the better response obtained from the sIgD⁺ population. The same degree of tolerance susceptibility was found in sIgD⁺ and sIgD^- populations from either 2-week-old or adult mice. The conclusion from these observations is that the T-independent response is not restricted to sIgD^- B cells and also that resistance to tolerogenesis in a T-independent assay system is not caused by acquisition of IgD.

Footnotes

¹ This work was supported by the National Health and Medical Research Council, Canberra, Australia, and by Grant A1-0-3958 from the National Institute for Allergy and Infectious Diseases, United States Public Health Service.