HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Dosch, H.-M. Articles by Gelfand, E. W. Search for Related Content PubMed Articles by Dosch, H.-M. Articles by Gelfand, E. W.

Role of Surface IgM and IgD in the Functional Differentiation of Human B Lymphocytes: Effect of Papain Treatment¹

From the Department of Immunology, The Research Institute, Hospital for Sick Children, 555 University Avenue, M5G 1X8, Toronto, Ontario, Canada

Abstract

Short-term treatment of normal human B lymphocytes with low concentrations of papain resulted in selective and reversible removal of sIgD determinants, whereas HLA and Ia-like antigens, sIgM as well as receptors for E, C3, and Fc_IgG were unaffected. When studied for their capacity to generate antigen-specific direct PFC, papain-treated (^-) B cells were highly sensitive to inactivation by even low concentrations of antigen. In addition, these cells were impaired in their ability to cooperate normally with T-helper cells or their humoral product(s).

Footnotes

¹ This study was supported by the Medical Research Council of Canada (MT 4875, MA 4611).

² B. Z. is a scholar of the Medical Research Council.

³ E. W. G. is recipient of a Queen Elizabeth II Scientist Award.