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A Serologic Comparison of Moloney Lymphoma Cell Surface and Moloney Oncornavirus Antigens¹

Abstract

Moloney lymphomas and Moloney sarcomas share strong tumor antigens. In this report we analyze the cell-surface antigens on a BALB/c Moloney lymphoma, LSTRA, using hyperimmune sarcoma regressor sera (Mo) as a primary reagent. We also use heterologous anti-viral p30 and gp70 sera for a direct analysis of virion protein antigens on the LSTRA surface. Using radiolabeled Mo-binding assays, we demonstrate that LSTRA tumor antigens detected by these sera are all Moloney viral antigens; approximately of these antigenic determinants are expressed on the intact virus, and the other determinants are revealed by detergent lysis of the virus. The major viral antigens expressed on the LSTRA cell surface are viral env gene products, whereas gag gene products are only sparsely represented. We conclude that Mo sera detect almost exclusively viral antigens on LSTRA cells, and these antigens are almost exclusively virion env gene products.

Footnotes

¹ This work was supported by grants from the American Cancer Society (IM56) and the United States Public Health Service (AI-09072 and GM-1922).

² Recipient of National Cancer Institute Postdoctoral Fellowship CA01186.

³ Medical Scientist Training Program Fellow (GM07365); present address, Center for Cancer Research Massachusetts Institute of Technology, Cambridge, Massachusetts.

⁴ Faculty Research Awardee, American Cancer Society. A portion of the work was completed while I. L. W. was a Josiah P. Macy Foundation Scholar. To whom correspondence and reprint requests should be sent.