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The Journal of Immunology, 1979, 123: 37-42.
Copyright © 1979 by The American Association of Immunologists, Inc.
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The Genetics of PLT Response

II. HLA-DRw Is a Major PLT-Stimulating Determinant1

William C. DeWolf2, Peter G. Carroll, Cyrus R. Mehta, Sharon L. Martin and Edmond J. Yunis

From the Sidney Farber Cancer Institute, Harvard Medical School, and Northeast Regional Red Cross Blood Program, Boston, Massachusetts 02115

Abstract

PLT response is restricted by the HLA-D region. The present study was undertaken to help define the role of HLA-DRw in PLT restimulation. Haplotype-primed intrafamily PLT cells were made against specificities HLA-Drw1, HLA-DRw3, and HLA-DRw7; each PLT was then restimulated with cells from a 35-member unrelated panel. Restimulation values for each PLT were subjected to bimodal clustering analysis. In addition, blocking experiments were performed with other intrafamily and homozygous typing cell PLT after preincubation with B cell alloantisera. The results show a high correlation (0.881 ≤ r ≤ 1.00) between the HLA-DRw specificity of the priming haplotype and the HLA-DRw specificity of unrelated panel cells that restimulate in PLT. When stimulating cells were absorbed with the corresponding DRw alloantisera or p29,34 heteroantiserum (against B cell specific antigens), PLT restimulation was significantly blocked. However, the PLT cells treated with antisera showed no effect. The results strongly suggest that HLA-DRw is the principal PLT-stimulating determinant.

Footnotes

1 This work was supported by research grants from the National Institutes of Health, National Cancer Institute: CA 19589, CA 20531, CA 09170, and American Red Cross Grant.

2 American Urologic Association Scholar.






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