The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

The Journal of Immunology, 1979, 123: 109-114.
Copyright © 1979 by The American Association of Immunologists, Inc.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kapp, J. A.
Right arrow Articles by Perlmutter, R. M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Kapp, J. A.
Right arrow Articles by Perlmutter, R. M.

Insulin-Specific Murine Antibodies of Limited Heterogeneity

I. Genetic Control of Spectrotypes1

Judith A. Kapp2, David S. Strayer3, Paul F. Robbins and Roger M. Perlmutter4

From the Department of Pathology and Laboratory Medicine, The Jewish Hospital of St. Louis and the Department of Pathology and Microbiology-Immunology, Washington University School of Medicine, St. Louis, Missouri 63110

Abstract

Immune responses by mice to porcine and bovine insulins are controlled by dominant genes linked to the H-2 gene complex. Mice bearing the H-2d haplotype respond to porcine insulin, whereas mice bearing other H-2 haplotypes do not; mice bearing the H-2b,d,v haplotypes respond to bovine insulin, whereas other strains do not. Analysis of serum antibody by isoelectric focusing demonstrates that antibodies specific for both porcine and bovine insulin are of restricted heterogeneity, suggesting that heterologous insulins stimulate a limited number of B cell clones. Banding patterns (spectrotypes) of sera from individuals of a given responder strain are remarkably uniform and these spectrotypic profiles are strain associated. In addition, phenotypic expression of spectrotypes is codominant in F1 hybrid mice and is controlled principally by allotype-linked genes. Analysis of cross-reactivity patterns demonstrates that all anti-porcine insulin antibodies bind bovine insulin and the majority of anti-bovine insulin antibodies bind porcine insulin. The minority of anti-bovine insulin antibodies that fails to bind porcine insulin may be specific for the A chain loop region of bovine insulin. These observations suggest that murine anti-insulin antibodies will provide a valuable model system for the identification of variable region genes that encode binding sites specific for protein molecules.

Footnotes

1 This work was supported by United States Public Health Service Grant AI-13987 from the National Institute of Allergy and Infectious Diseases and Biomedical Research Support Grant RR-0549 to The Jewish Hospital of St. Louis from the Division of Research Resources.

2 To whom correspondence should be addressed.

3 Supported by Training Program in Experimental Pathology Grant GM-0897 from the National Institutes of Health.

4 Supported by Medical Scientist Training Program Grant GM-02016.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1979 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1979 by The American Association of Immunologists, Inc. All rights reserved.