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Shared Idiotypes on Human Peripheral Blood B and T Lymphocytes¹

From the Institute of Immunology and Rheumatology, Rikshospitalet, The National Hospital, and National Institute of Public Health, Oslo, Norway

Abstract

In a patient with an IgG monoclonal serum component possessing anti-streptolysin O activity, we have demonstrated peripheral blood B and T lymphocytes with shared or similar idiotypes. The idiotypic T lymphocyte membrane structure was capable of binding the specific antigen (SLO). After radioiodination and subsequent detergent solubilization of the same T cell population, immunoprecipitation of the lysate by employing anti-idiotypic antibodies, resulted in the isolation of a polypeptide chain with a m.w. of 70,000 on SDS polyacrylamide gels under reducing conditions. The polypeptide expressed no isotypic immunoglobulin markers. Internal labeling experiments indicated that this membrane structure was actively synthesized by the T lymphocytes.

Footnotes

¹ This work has in part been presented at the IX Annual Meeting of the Scandinavian Society for Immunology, Stockholm, May 1978.

² Please address correspondence to: Tor Lea, Institute of Immunology and Rheumatology, F. Qvamsgt. 1, Oslo 1, Norway.