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Characteristics of in Vitro Production of Antibodies to DNA in Normal and Autoimmune Mice¹

From the Department of Medicine, University of California, San Francisco, and the Arthritis and Immunology Section, Veterans Administration Hospital, San Francisco, California

Abstract

The in vitro production of antibodies to dsDNA was studied with spleen cells from normal and autoimmune mice. After culture for 4 days, the binding of dsDNA in the culture supernatant was measured by a radioimmunoprecipitation assay. The production of antibodies to dsDNA by spleen cells appeared at 15 hr after culture and reached a plateau at 24 hr. No antibodies were produced by thymus cells or splenic T cells. The specificity for dsDNA was shown by competitive inhibition with nonradioactive nucleic acids. Autoimmune strains of mice (NZB/NZW, BXSB, MRL/1) produced more antibodies to dsDNA than did several control strains. Young B/W mice and control strain mice produced mainly IgM antibodies, whereas older B/W mice produced predominantly IgG antibodies to dsDNA. The in vitro production of antibodies to dsDNA by aged B/W spleen cells was macrophage and T cell dependent.

Footnotes

¹ This work was supported by the Medical Research Service of the Veterans Administration, by the Department of Health, State of California, Grant 76-57090, and by United States Public Health Service Grant AM 16140.

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