HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Katz, D. H. Search for Related Content PubMed Articles by Katz, D. H.

Adaptive Differentiation of Murine Lymphocytes

III. T and B Lymphocytes Display Reciprocal Preference for One Another to Develop Optimal Interacting Partner Cell Sets^1,2,

From the Department of Cellular and Developmental Immunology, Scripps Clinic and Research Foundation, La Jolla, California 92037

Abstract

Responses to the synthetic terpolymer L-glutamic acid, L-lysine, L-phenylalanine (GL) and hapten derivatives thereof are controlled by two complementing H-2-linked Ir genes in the mouse. F₁ hybrids derived from two different nonresponder strains (one of which possesses the and the other Ir-GL gene) are phenotypic responders to GL and 2,4-dinitrophenyl (DNP)-GL. Moreover, spleen cells from DNP-GL-primed F₁ mice can adoptively transfer secondary anti-DNP antibody responses to irradiated F₁ recipients that have been challenged with DNP-GL. When, however, GL-primed F₁ helper T cells are transferred together with the DNP-specific F₁ B cells that had been primed in separate mice altogether by DNP coupled to an unrelated protein carrier, such mixtures failed to develop adequate adoptive secondary anti-DNP responses to DNP-GL. This contrasted with the ability of the same GL-primed F₁ T cells to provide helper activity for DNP-primed B cells from responder recombinant B10.A (5R) mice. More important, the apparent defect of GL-primed F₁ T cells in providing help for DNP-primed F₁ B cells (primed to a DNP-protein conjugate) could be readily overcome by using DNP-primed B cells from donor F₁ mice primed with DNP-GL. As discussed herein, these results suggest that interacting T and B lymphocytes pair off into partner cell sets, any pair of which interact optimally when a "best fit" reciprocal self-recognition occurs between them.

Footnotes

¹ This is Publication No. 93 from the Department of Cellular and Developmental Immunology and Publication No. 1683 from the Immunology Departments, Scripps Clinic and Research Foundation, La Jolla, California.

² This work was supported by United States Public Health Service Grant AI-13781, National Foundation Grant 1-540, and Biomedical Research Support Grant RRO-5514.