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The Molecular Identification of Two Serologically Defined Gene Products of the Rat Major Histocompatibility Complex¹

From the Departments of Pathology, Hokkaido University School of Medicine, Sapporo, 060, and Asahigawa Medical College, Asahigawa, 071-01, Japan

Abstract

The two SD gene products of the rat major histocompatibility complex were identified by immunochemical means with rat alloantisera. WKA anti-TO antiserum contained anti-TO reactivity possibly detecting a unique private specificity for the Tokyo strain or rats, as well as some other cross-reactivity against common public specificities among ACI, Spd, LEJ, and TO. WKA anti-TO antiserum could react with ¹²⁵I-labeled AgB⁴ preparation. The immunoprecipitates gave two peaks of radio-activity on SDS-PAGE corresponding to positions of 37,000 and 12,000 daltons, respectively, showing the basic structure of AgB antigens as described previously. These components were confirmed, by a sequential immunoprecipitation test with rabbit anti-H-2K,D and rabbit anti-HLA-A,B antiserum, to be of the rat major histocompatibility antigens. Two different SD gene products were identified by sequential immunoprecipitation tests in which ¹²⁵I-labeled AgB⁴ preparation was first treated with WKA anti-TO antiserum and the residual supernatants were allowed to react with a test antiserum, TO anti-ACI antiserum. Pretreatment of the antigen with WKA anti-TO antiserum could not remove the antigen molecules reactive with TO anti-ACI antiserum. Conversely, pretreatment of the antigen preparation with TO anti-ACI antiserum removed concomitantly the molecules detectable by WKA anti-TO antiserum, suggesting that both molecules carry the same private and/or public specificity(ies). The two SD antigens were inherited together in backcross animals of (ACI x WKA)F₁ x WKA, indicating a close linkage of the two SD loci. There was one discrepant animal out of 63 rats tested, suggesting a dissociation of the two loci.

Footnotes

¹ This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan.