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Macrophage Cell Lines Produce a Cytotoxin

From the Immunopathology Section, Laboratory of Immunobiology, National Cancer Institute; and Laboratory of Microbial Immunity, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

Abstract

Cytotoxic activity, measured by release of tritium from ³H-(methyl)-thymidine-labeled 7943 cells, was found in culture fluids from four of five macrophage cell lines. In the P388D1 cell line, cytotoxic activity was highest after 7 days of culture, 2 days after the cells reached confluence. The activity was not lost upon dialysis or lyophilization. When culture fluids were chromatographed on Sephadex G-200, two activity peaks were obtained: one in the void volume and the other corresponding to a m.w. of 40,000 to 50,000.