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From the Department of Internal Medicine, Uniformed Services University of the Health Sciences, and the Experimental Pathology Department, Naval Medical Research Institute, Bethesda, Maryland 20014
Abstract
Rhesus monkey spleen, mesenteric lymph node, peripheral lymph node, blood, thymus, and bone marrow mononuclear cell suspensions were stained with fluorescein-conjugated F(ab')2 fragments of affinity-purified rabbit anti-human Fab, rabbit anti-human 
, rabbit anti-human µ, rabbit anti-human 
, and/or rabbit anti-human 
as well as rabbit anti-keyhole limpet hemocyanin (RaKLH) and analyzed for fluorescence intensity and frequency of positive cells with a fluorescence-activated cell sorter. Greater than 90% of surface (s)IgM-bearing spleen, blood, and lymph node lymphocytes bore sIgD, whereas only approximately 40% of sIgM-bearing bone marrow lymphocytes had sIgD. In all of these organs greater than 90% of sIgD-bearing cells also had sIgM. Substantially smaller numbers of lymphocytes bore sIgG than sIgM or sIgD, and most sIgG-bearing cells in all organs studied lacked sIgM and sIgD. Few if any cells in any organ studied bore detectable sIgA, and less than 1% of thymus cells had any sIg.
B cell populations from spleen, peripheral lymph node, and mesenteric lymph node averaged similar quantities of sIgM and sIgD per B cell. Blood B cells averaged twice as much sIgM and sIgD as B cells from spleen and lymph node, and 9 times as much sIgD as sIgD+ bone marrow cells. sIgD+ bone marrow B lymphocytes have considerably more sIgM than sIgD- sIgM+ bone marrow B cells. Our data confirm that the sIgD+ sIgM+ cells are the predominant B lymphocyte in primates, and suggest that monkey B cells acquire sIgD in the marrow before migrating to other lymphoid organs.
Footnotes
1 This work was supported by the Naval Medical Research and Development Command, Work Unit No. M0095-PN.001-1030, and the Uniformed Services University of the Health Sciences Protocol Nos. R08307 and R08308. The opinions or assertions contained herein are the private ones of the authors and are not to be construed as official or reflecting the views of the Department of Defense, the United States Navy Department, or the naval service at large. The experiments reported herein were conducted according to the principles set forth in the "Guide for the Care and Use of Laboratory Animals," Institute of Laboratory Animal Resources, National Research Council, Department of Health, Education and Welfare Publication No. (National Institutes of Health) 74-23.
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