HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Zinkernagel, R. M. Articles by Althage, A. Search for Related Content PubMed Articles by Zinkernagel, R. M. Articles by Althage, A.

Search for Suppression of T Cells Specific for the Second Nonhost H-2 Haplotype in F₁ P Irradiation Bone Marrow Chimeras¹

From the Department of Immunopathology; Scripps Clinic and Research Foundation, La Jolla, California 92037

Abstract

Irradiation bone marrow chimeras were made by lethally irradiating parental (P) mice and reconstituting them with T cell-depleted bone marrow cells from F₁ hybrid offsprings. These F₁ P chimeric lymphocytes were then analyzed for suppressor mechanisms that could cause the differentiation of effector T cells whose responsiveness was restricted in specificity to the host parent's H-2 haplotype. No evidence was detected for acute or chronic suppression of the second parent's restriction specificity in the following tests of cytotoxic T cell activity against virus-infected targets: 1) Mixing normal F₁ spleen cells with various amounts of chimeric spleen and lymph node cells during the cytotoxicity test or, during sensitization in F₁ mice tested 5 days after irradiation and infection, revealed no suppression of the activity for the second parental type target cells. 2) Chimeric F₁ P₁ lymphocytes did not suppress alloreactivity directed against the second parental H-2 type. 3) Presence or absence of T cells in the transferred, reconstituting F₁ bone marrow cells did not change the hosts' influence on the selection of restriction specificity. 4) F₁ P chimeric lymphocytes transferred to 500 R irradiated normal F₁ mice did not suppress reactivity for the second parental type targets. 5) F₁ P chimeric lymphocytes or bone marrow cells transferred to lethally irradiated F₁ could still differentiate to express the second parental restriction specificity; similarly, when F₁ P lymphocytes were mixed with normal but anti- plus complement-treated F₁ bone marrow cells and transferred to lethally irradiated F₁, both restriction specificities were generated.

Therefore, F₁ P irradiation bone marrow chimeras as described and tested here do not contain mechanisms that suppress the restriction specificity of the second parent, which is not a host to the maturing F₁ T cells, at least at the effector stage or during differentiation.

Footnotes

¹ This is Publication No. 1650 from the Department of Immunopathology, Scripps Clinic and Research Foundation, La Jolla, California 92037. This work was supported by United States Public Health Service Grants A1-13775 and A1-00273.