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Enhanced Uptake of ³H-Glucosamine by Macrophages Stimulated by Macrophage-Activating Factor¹

From the Departments of Medicine and Dermatology, Harvard Medical School and Robert B. Brigham Hospital, Boston, Massachusetts 02115

Abstract

The enhanced uptake and incorporation of ³H-glucosamine by guinea pig peritoneal exudate macrophages stimulated by macrophage-activating factor (MAF) were studied. MAF was produced by concanavalin A- (Con A) stimulated lymphocytes. Two systems were developed to assay this phenomenon, one using microtiter plates, the other using suspension cultures. Enhanced uptake of ³H-glucosamine by macrophages was found after 48-hr incubation in microtiter plates and after 24 hr in suspension cultures. Increased uptake of ¹⁴C-glucose and ³H-galactose was also observed under these conditions. The enhanced uptake of ³H-glucosamine was markedly reduced or abolished by the addition of glucose to the cell cultures or by replacement of the medium with fresh medium before pulsing with labeled sugar. These observations indicate that the mechanism of enhanced uptake reflects in part increased glucose utilization by the stimulated macrophages.

Footnotes

¹ This work was supported in part by National Institutes of Health Grant AI-07685 and NCI Contract N01-CB-53869.

² Dr. Sober was a trainee on National Institutes of Health AI-00366.