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Studies of the Mechanisms for the Induction of in Vivo Tumor Immunity

IV. Enhancement of the in Vitro Generation of Secondary Cell-Mediated Cytotoxic Response by Normal Peritoneal Macrophages and Their Culture Supernatants¹

From the Laboratory of Cell Biology, National Cancer Institute, Bethesda, Maryland 20014, and IIIrd Department of Internal Medicine, Osaka University Medical School, Fukushima, Osaka 553, Japan

Abstract

The effect of macrophages on the induction of the cell-mediated cytotoxicity against a leukemia in a syngeneic system was investigated. The addition of exogenous peritoneal cells from normal C57BL/6 mice enhanced the in vitro secondary cell-mediated cytotoxic response of both spleen and lymph node cells as responding cells against syngeneic FBL-3 leukemia. Peritoneal phagocytic macrophages seemed to be responsible for the enhancement. No inhibitory effect was demonstrated by the addition of peritoneal macrophages at a concentration as high as 20%, whereas the primary cytotoxic allograft response was significantly suppressed. In the present studies, there was no absolute restriction of macrophage-T cell interaction by an H-2 barrier. Supernatants of peritoneal macrophage cultures also enhanced this cell-mediated cytotoxic response. There was no difference between the effects of syngeneic or allogeneic peritoneal macrophage culture supernatants.

Footnotes

¹ This work was supported in part by a grant from the Japan Society for Promotion of Science.

² All correspondence should be addressed to Dr. igarashi, Osaka University Medical School, Fukushima, Osaka 553, Japan. Recipient of the Japan Society for Promotion of Science Grant on US-Japan Cooperative Cancer Research Program.