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The Journal of Immunology, 1979, 122: 997-1001.
Copyright © 1979 by The American Association of Immunologists, Inc.
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beta2-Microglobulin Enhances Human Lymphocyte Surface Receptor Expression for IgG1

Ruth E. Birch, Michael W. Fanger and George M. Bernier

From the Departments of Medicine and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106; and the Department of Medicine, Dartmouth Medical School, Hanover, New Hampshire 03755

Abstract

Because of its close sequence homology to immunoglobulin constant domains, the effect of beta2-microglobulin (beta2µ) on the expression of Fc receptors on human peripheral blood lymphocytes was studied. Treatment of peripheral blood lymphocytes (PBL) at 4°C with beta2-microglobulin resulted in an increase in the percentage of lymphocytes expressing receptors for the Fc portion of IgG (Fc{gamma}) from 34% in untreated preparations to 54% in preparations incubated with 50 µg of beta2µ/2 x 106 cells. As shown by the binding of 125I-labeled beta2µ, the molecule was taken up by peripheral lymphocytes and was associated with those lymphocytes that expressed Fc{gamma} receptors. beta2µ treatment had no demonstrable effect on the expression of other lymphocyte markers—E, EAC, sIg. The increase in Fc{gamma} receptor expression was observed on T cells. Removal of adherent cells had no effect on beta2µ induced Fc{gamma} receptor expression, but the increased Fc{gamma} receptor expression was abrogated by treatment with rabbit anti-human beta2µ F(ab')2 fragments. These findings suggest that high concentrations of beta2µ exert effects on lymphocyte surfaces that may have functional as well as structural consequences in regulation of the immune system.

Footnotes

1 This work was supported by Grants AM 14700, CA 17864, and AI 10148 from the United States Public Health Service.






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