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Detection of HLA-DRw (Ia-Like) Antigens on Human T Lymphocytes Grown in Tissue Culture¹

From the Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710, the Laboratory of Immunodiagnosis, National Cancer Institute, Bethesda, Maryland 20014, and the Department of of Cell Biology, Litton Bionetic, Inc., 7300 Pearl Street, Bethesda, Maryland 20014

Abstract

Human T lymphocytes that proliferate in the presence of conditioned medium from PHA-stimulated allogeneic peripheral blood cells were shown to express Ia antigens after the 8th culture day. Ia antigens as detected by xenogeneic antisera were present on 80 to 90% of the cultured cells which were also strongly reactive with xenogenic antisera defining a human T cell antigen and formed E rosettes. Control cultures with PHA or no conditioned medium expressed T cell but not Ia antigens. These cultured T cells also express the same HLA-DRw determinant as the B cells of the donor they were derived from. Absorption of xenogeneic Ia, and HLA-DRw alloantisera with cultured T cells completely removed the reactivity of these sera for enriched peripheral blood B lymphocytes from normal donors.

Footnotes

¹ This work was supported by Grant AM 08054 from the National Institute of Arthritis, Metabolism and Digestive Diseases.

² Attaché de Recherche INSERM.