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Failure of NZB Spleen to Respond to Prethymic Bone Marrow Suppressor Cells¹

From the Department of Medicine, University of California, San Francisco, and the Section of Clinical Immunology, Veterans Administration Hospital, San Francisco, California 94121

Abstract

Mouse bone marrow contains -negative lymphocytes that can suppress an in vitro plaque response by spleen cells primed in vivo with burro red blood cells (BRBC). These bone marrow cells are radiosensitive and can be induced with thymosin fraction 5 or ₁ thymic peptides to express the antigen. Enrichment for these suppressor pre-T lymphocytes can be achieved by a one-step density centrifugation, macrophage depletion, or a combination of both procedures.

NZB mice, which spontaneously develop an autoimmune disorder, have a suppressor abnormality revealed by this assay system. Upon analysis, they have normal BM pre-T suppressor cells but their spleen cells are refractory to the BM suppressor signal. NZB BM suppressor cells inhibit the response by DBA/2 spleen cells, but DBA/2 BM suppressor cells do not inhibit NZB spleen. This resistance to suppression is a property of the B cell fraction recovered from NZB spleen.

Footnotes

¹ This work was supported by the Medical Research Service of the Veterans Administration and by United States Public Health Service Grants CA-22518 and AG-00911 from the National Institutes of Health.