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Genetic Control of the Immune Response to Staphylococcal Nuclease

IX. Recombination Between Genes Determining BALB/c Antinuclease Idiotypes and the Heavy Chain Allotype Locus

From the Transplantation Biology Section, Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014, and Litton Bionetics, Inc., Kensington, Maryland 20014

Abstract

The genetic linkage relationship of two antinuclease idiotypes produced by the BALB/c strain was investigated in the backcross (BALB/c x CB.20) x CB.20. These two idiotypes were detected by Lewis rat anti-idiotypic antisera prepared against affinity-purified A/J and SJL antinuclease antibodies, termed the A/J and SJL idiotypes, respectively. Both idiotypes were found to be linked to the IgCH^a immunoglobulin heavy chain allotype locus. There was, however, a high frequency of recombination observed between both markers and the IgCH^a locus, with eight of 83 backcross animals recombinant for the A/J idiotype and five of 83 recombinant for the SJL idiotype. All such recombinant animals were IgCH^b/b homozygotes that had gained one or both idiotypes. These results are consistent with a genetic map of V_H region genes in the BALB/c strain in which genes determining the SJL idiotype are closer to the IgCH^a allotype locus than are genes determining the A/J idiotype. This high frequency of recombination may indicate that the chromosome segment containing V_H region genes is very large or that it has structural features that promote recombination.