HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lake, A. M. Articles by Walker, W. A. Search for Related Content PubMed Articles by Lake, A. M. Articles by Walker, W. A.

Intestinal Goblet Cell Mucus Release

II. In Vivo Stimulation by Antigen in the Immunized Rat¹

From the Departments of Pediatrics, Medicine and Anatomy, Harvard Medical School and the Pediatric Gastrointestinal Unit, Children's Service; and the Clinical Immunology and Allergy Unit Medical Service, Massachusetts General Hospital, Boston, Massachusetts 02114

Abstract

We previously reported that the infusion of certain soluble immune complexes stimulated mucus release from the rat small intestine in vivo. The present studies sought to evaluate the response of the intestine of normal and immunized rats to the infusion of antigen alone. One hour after the intraduodenal infusion of antigen, small intestinal washings were obtained and analyzed for the presence of ³⁵S-labeled, high m.w. glycoprotein of goblet cell origin. The amount of goblet cell glycoprotein released was estimated from the radioactivity present in the void volume of a Sepharose 4B gel filtration column.

The release of goblet cell mucus was enhanced by antigen stimulation in orally immunized animals. The discharge of goblet cell mucus was not increased after antigen infusion in animals immunized by the i.p. route despite the induction of high levels of serum antibody. The inability to demonstrate release of mucus after antigen challenge in systemically immunized rats suggests that the amount or the type(s) of antibody required at the mucosal surface is produced only after oral immunization.

Footnotes

¹ This work was supported by grants from the National and Massachusetts Chapters of the Arthritis Foundation, the L. H. Bendit Foundation, the National Institutes of Health (AM-21505, AM-16269, and AM 23099-1), United States Army Medical Research and Development Command (DADA-C-17-70-0113), and the National Foundation for Ileitis and Colitis, Inc.

² Address correspondence to: Alan M. Lake, M.D., Pediatric Gastrointestinal Unit, Massachusetts General Hospital, Boston, Massachusetts 02114.

This article has been cited by other articles:

				P R Gibson and J G Muir Reinforcing the mucus: a new therapeutic approach for ulcerative colitis? Gut, July 1, 2005; 54(7): 900 - 903. [Full Text] [PDF]

				C. POTHOULAKIS, I. CASTAGLIUOLO, and S. E. LEEMAN Neuroimmune Mechanisms of Intestinal Responses to Stress: Role of Corticotropin-Releasing Factor and Neurotensin Ann. N.Y. Acad. Sci., May 1, 1998; 840(1): 635 - 648. [Abstract] [Full Text] [PDF]