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Idiotypic Analysis of Anti-Gat Antibodies

III. Determinant Specificity and Immunoglobulin Class Distribution of CGAT Idiotype¹

From the Department of Pathology, Harvard Medical School, 25 Shattuck Street, Boston, Massachusetts 02115

Abstract

The humoral response to poly-(L-glutamic acid⁶⁰, L-alanine³⁰, L-tyrosine¹⁰), GAT, in mice is further characterized by both idiotype and fine specificity analyses. The common idiotype on murine anti-GAT antibodies (CGAT) was identified in anti-GAT antisera from seven additional strains of mice. These data confirm that the CGAT idiotype can be induced in all inbred strains of mice.

Using a partially inbred strain of mice selected for their ability to respond to poly-(L-glutamic acid⁵⁰, L-tyrosine⁵⁰), GT, we demonstrated that the GT copolymer is capable of inducing antibodies that express the CGAT idiotype. In contrast, antisera directed against poly-(L-glutamic acid⁶⁰, L-alanine⁴⁰), GA, bind GAT but lack CGAT idiotype. These results indicate that GAT molecules contain determinants either similar or identical to those on GT molecules, which are responsible for the induction of CGAT idiotypic antibodies. We also demonstrated that both GAT responder and nonresponder strains of mice can produce anti-GAT antibodies with similar fine specificity patterns and CGAT idiotype. In addition, we demonstrate that antibodies of the IgM, IgG1 and IgG2 immunoglobulin classes express the CGAT idiotype.

Footnotes

¹ This work was supported by Grants AI-13419 and AI-00152 from the National Institutes of Health and by Grant PCM 75-22422 from the National Science Foundation.