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In Vitro Sensitization of Human Lymphocytes Against Histiocytic Lymphoma Cell Lines

III. The Activity of Culture-Induced Suppressor Cells¹

Cancer Biology Research Laboratory, Department of Radiology, Stanford University School of Medicine, Stanford, California 94305

Abstract

Normal PBL were sensitized in vitro against an allogeneic diffuse histiocytic lymphoma cell line and their activity was measured by radiolabel release from target cells. We have reported earlier that a non-T cell population, found among the PBL, was responsible for inhibiting in vitro sensitization. In the present work we found that culturing PBL in vitro caused the induction of radioresistant suppressor cells which affected the sensitization phase of the in vitro response. The culture-induced suppressor cells had macrophage-like characteristics. The activity of the suppressor cells depended on an additional helper population that was adherent to nylon wool, but did not adhere to plastic and was nonphagocytic. The cooperation of these two different cell populations was required for the expression of suppressive activity.

Footnotes

¹ This work was supported by research contract N01-CB-63988 from the National Cancer Institute, National Institutes of Health, Bethesda, Maryland.

² Present address: Department of Oncology, Sharett Institute of Oncology, Hebrew University-Hadassah Medical Center, Jerusalem, Israel.

³ Present address: 182 South Bedford Road, Chappaqua, New York 10514.