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Characterization of in Vitro T-Lymphocyte Colonies from Spleens of Nude Mice¹

Ontario Cancer Institute, and Department of Medical Biophysics, University of Toronto, 500 Sherbourne Street, Toronto, Ontario, Canada M4X 1K9

Abstract

Cells residing in the spleens of nude mice that appear Thy-1 negative can give rise to Thy-1 positive colonies in an in vitro culture system. Cells from pooled colonies can provide an accessory cell required for the development of cytotoxic lymphocytes from their precursors in an in vitro mixed lymphocyte reaction. As few as 50 such colony cells produce detectable activity.

Footnotes

¹ This work was supported by the Ontario Cancer Treatment and Research Foundation and by the National Cancer Institute of Canada.

² Address for reprints: Dr. R. G. Miller, Ontario Cancer Institute, Division of Biological Research, 500 Sherbourne Street, Toronto, Ontario, Canada M4X 1K9.