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Primary in Vitro Cytotoxic Response of F₁ T Lymphocytes against Parental Antigens¹

Department of Biology, University of California, San Diego, La Jolla, California 92093

Abstract

Spleen cells from adult female (AKR/J x BALB/c)F₁ mice can respond to mitomycin C-treated spleen from AKR/J mice and can generate effector CTL in a 5-day primary in vitro culture. The response is comparable in magnitude to the response to allogeneic H-2K or H-2D antigens. The response is T cell mediated and is directed to antigen(s) present only on the parental cells. The target cell must be homozygous at H-2K^k to be lysed and H-2D^k antigens do not serve as a target in this response. Spleen cells from (B10.BR x B10.D2) hybrids that have been stimulated with AKR/J lyse B10.Br as well as AKR/J target cells. Similar H-2^k/d hybrid F₁ anti-H-2^k parent responses are seen in certain other strain combinations. A number of possible interpretations of these responses are discussed.

Footnotes

¹ This work was supported by National Institutes of Health Grant AI-08795 and American Cancer Society IM-1K.

² Dr. Ishikawa is supported partially by Keio University, Tokyo, Japan.