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Immunochemical Characterization of the Ly-8.2 Murine Lymphocyte Alloantigen: Possible Relationship to Actin¹

From the Banting and Best Department of Medical Research and Department of Microbiology and Parasitology, University of Toronto, Toronto, Canada M5G 1L6 and Department of Microbiology, University of Southern California Medical School, Los Angeles, California 90033

Abstract

An immunochemical analysis of the Ly-8.2 antigen was performed. Reactivity of B10-radiolabeled T and B cell lysates with a C3H anti-AKR serum immunoprecipitates a major 43,000 m.w. protein detectable by one-dimensional gel electrophoresis. This protein can be radioiodinated, however, it does not appear to bind to lentil lectin. Two-dimensional gel electrophoresis and peptide-mapping studies indicate that this protein is structurally very similar to lymphocyte actin. The possible molecular relationship between Ly-8.2, other lymphocyte membrane antigens, and actin is discussed.

Footnotes

¹ This work was supported by the Medical Research Council of Canada Grants MA 5729 and MA 6004, NSF Grant PCM77-02722, and by stipends from the Dean's Fund, Faculty of Medicine, University of Toronto, to the medical undergraduate summer students, Ms. S. Friedman and Mr. A. Fegelman.

² Please address reprint requests to Dr. T. L. Delovitch, Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario, Canada, M5G 1L6.