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Characterization of an Antiserum Specific for the Fab Fragment

Its Use for Detection of -Heavy Chain Disease Protein by Immunoselection¹

From the Department of Immunopathology, Scripps Clinic and Research Foundation, La Jolla, California 92037

Abstract

An antiserum to the Fab fragment of human IgA was prepared by injecting - and -type IgA1 myeloma protein Fab fragments in complete Freund's adjuvant into a goat. After appropriate absorptions, the antiserum reacted specifically with Fab fragments, normal IgA, and - and -type IgA1 and IgA2 myeloma proteins. This antiserum contained predominantly antibodies to determinants shared by different IgA myeloma proteins and expressed on isolated chains. These determinants were presumably located on the first constant domain of the chain (Fd fragment). When incorporated into agarose, the anti-Fab antiserum is a potentially valuable reagent for screening human sera for -heavy chain disease proteins by an immunoelectrophoretic method of immuno-selection.

Footnotes

¹ This work was supported by United States Public Health Service Grants HL-21565, HL-16411, and AI-10734, and Biomedical Research Support Program Grant RRO-5514. This is Publication No. 1576 from the Scripps Clinic and Research Foundation.

² Present address: Department of Medicine, University of Sydney at Royal North Shore Hospital, St. Leonards 2065, New South Wales, Australia.