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Specific Antibodies to Hemoglobin A₁ (anti-Glu) and Hemoglobin S (anti-Val) in the Guinea Pig: Immunologic and Structural Correlations¹

From the Departments of Medicine and Biochemistry, State University of New York at Buffalo School of Medicine, Veterans Administration Hospital, Buffalo, New York 14215

Abstract

Like goats and sheep, guinea pigs can produce, in response to human sickle cell hemoglobin (⁶ Glu Val), an antibody population (anti-Val) that will bind sickle cell hemoglobin but not normal hemoglobin HbA. Unlike goats and sheep, guinea pigs can produce in response to human hemoglobin A₁ an antibody fraction, anti-Glu, that will not react with human sickle cell hemoglobin. These anti-Glu antibodies have been isolated by affinity chromatography and their specificity confirmed by fluorescence-quenching titrations. The sequence of the first 10 amino acids of the -chain of guinea pig hemoglobin has been determined. This sequence differs from those of both hemoglobin HbA and sickle cell hemoglobin by two residues, those at positions 5 and 6. This explains the similarity of the immunogenicity of this site on the two human hemoglobins when administered to guinea pigs. Both goats and sheep are identical to hemoglobin A₁ at the -6 position.

Footnotes

¹ This work was supported by Grant HL 12524 from the United States Public Health Service Heart Lung and Blood Institute, and by research funds from the Veterans Administration.

² Present address: Department of Immunology, Albert Einstein College of Medicine, Bronx, New York.

³ Established investigator of the American Heart Association.

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