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Development of IgE-Forming Cells in Vitro from Rat Mesenteric Lymph Node Cells¹

From the Department of Medicine, The Johns Hopkins University School of Medicine at the Good Samaritan Hospital, Baltimore, Maryland 21239

Abstract

Mesenteric lymph node cells from normal rats and rats infected with Nippostrongylus brasiliensis (Nb) were cultured with pokeweed mitogen (PWM) or Nb antigen, and the development of IgM-, IgG2a-, or IgE-containing cells was assessed by immunofluorescence. Normal lymph node cells stimulated with PWM developed into both IgM- and IgE-containing cells, whereas similar stimulation of cells from Nb-infected rats resulted in the development of IgM-, IgG2a-, and IgE-containing cells. The in vitro plasma cell response to PWM was dependent on the presence of T lymphocytes. Lymph node cells from Nb-infected rats responded to Nb antigen and developed into plasma cells of IgM, IgG, and IgE classes. The response was antigen specific and required antigen-primed T cells. Depletion of IgE-bearing cells of IgM-bearing cells before stimulation with either PWM or Nb antigen diminished the level of IgE-forming cell development, suggesting that IgE-IgM double bearing cells are precursors of IgE-forming cells. The distribution of the three isotypes among the Ig-forming cells that developed in response to PWM was influenced by the source of both B and T cells. When B cells from Nb-infected rats were employed as a source of precursors, T cells from infected animals were more effective than normal T cells for the development of IgE-forming cells, whereas the latter cells were more effective for the development of IgG2a-forming cells than T cells from infected animals.

Footnotes

¹ This work was supported by Research Grants AI-11202 and AI-10060 and AI-14784 from the United States Public Health Service. This paper is publication No. 323 from the O'Neill Laboratories at the Good Samaritan Hospital.

² Postdoctoral trainee supported by Training Grant T32, AI-07056, from the United States Public Health Service.