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Cell Surface Glycoproteins of Rat Lymphocytes

I. Correlation of Mitogenic Stimulation by Periodate or Neuraminidase and Galactose Oxidase with the Presence of Papain-Sensitive Glycoproteins¹

From The Rockefeller University, New York 10021

Abstract

Oxidation of viable rat lymph node lymphocytes with either periodate or a combination of neuraminidase and galactose oxidase (NGO), followed by reduction with tritiated sodium borohydride, labels similar sets of cell-surface molecules as assessed by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis. Periodate and NGO induce blast transformation of lymph node lymphocytes (oxidative mitogenesis), and borohydride reduction inhibits the proliferative response. Thus, it is inferred that some or all of the glycoproteins that are labeled with tritiated borohydride may be involved in mediating the stimulation caused by the oxidizing agents.

Treatment of lymph node lymphocytes with 5 units/ml papain abolishes the response to periodate or NGO but does not significantly affect the response to Con A. At the same time, papain treatment eliminates the labeled bands representing six high m.w. glycoproteins (175,000, 170,000, 160,000, 155,000, 100,000, and 70,000 daltons). No significant effect is seen on the labeling of the other components visualized in the slab gels. The results implicate the subset of six high m.w. papain-sensitive sialoglycoproteins in mediating oxidative mitogenesis of rat lymph node lymphocytes.

Footnotes

¹ This work was supported by United States Public Health Service Grant Ca-16875, by National Science Foundation Grant PCM 76-16657, and by the Environmental Protection Agency under Contract No. 68-02-2420.