HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Baker, P. E. Articles by Smith, K. A. Search for Related Content PubMed Articles by Baker, P. E. Articles by Smith, K. A.

The Effect of T Cell Growth Factor on the Generation of Cytolytic T Cells¹

From the Hematology Research Laboratory, Department of Medicine, Dartmouth Medical School and the Norris Cotton Cancer Center, Hanover, New Hamsphire, 03755

Abstract

The development of cytotoxic effector cells through primary allogeneic mixed tumor-lymphocyte culture (MTLC) was found to be accompanied by the production of T cell growth factor (TCGF). Addition of supplemental TCGF to MTLC resulted in the generation of significantly greater quantities of effector cells, and these effector cells displayed augmented cytotoxic activity. The TCGF-induced effect could not be duplicated by the addition of fresh medium or a mitogenic concentration of concananvalin A. Although TCGF augmented the proliferation of antigen-nonreactive cells, antigen-reactive cells appeared to be preferentially stimulated by TCGF. Finally, it was shown that depletion of TCGF from MTLC resulted in an impairment of proliferation and differentiation of cytotoxic effector cells. These findings demonstrate that soluble factors are involved in the regulation of in vitro cell-mediated immune responses in an analogous manner to similar factors that have been shown to regulate humoral immune responses. Therefore, the forces affecting TCGF production may modulate the amplitude of a T cell-mediated cytolytic response.

Footnotes

¹ This work was supported in part by NCI Grant RO1-17643-03, NCI Contract No. NO1-CB-74141, NCI Contract No. NO1-CN-55199, and a grant from the National Leukemia Association, Inc.

This article has been cited by other articles:

				H. Kojima, Y. Kanno, H. Hase, and T. Kobata CD4+CD25+ Regulatory T Cells Attenuate the Phosphatidylinositol 3-Kinase/Akt Pathway in Antigen-Primed Immature CD8+ CTLs during Functional Maturation J. Immunol., May 15, 2005; 174(10): 5959 - 5967. [Abstract] [Full Text] [PDF]

				H. Dooms, E. Kahn, B. Knoechel, and A. K. Abbas IL-2 Induces a Competitive Survival Advantage in T Lymphocytes J. Immunol., May 15, 2004; 172(10): 5973 - 5979. [Abstract] [Full Text] [PDF]

				Y. Zhan, A. J. Corbett, J. L. Brady, R. M. Sutherland, and A. M. Lew CD4 Help-Independent Induction of Cytotoxic CD8 Cells to Allogeneic P815 Tumor Cells Is Absolutely Dependent on Costimulation J. Immunol., October 1, 2000; 165(7): 3612 - 3619. [Abstract] [Full Text] [PDF]

				K. Smith Interleukin-2: inception, impact, and implications Science, May 27, 1988; 240(4856): 1169 - 1176. [Abstract] [PDF]