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Characterization of a B Cell Defect in the NZB Mouse Manifested by an Increased Ratio of Surface IgM to IgD¹

From the Departments of Internal Medicine and Microbiology, University of Texas Southwestern Medical School, Dallas, Texas 75235

Abstract

In an effort to define the cellular basis of abnormalities in polyclonal B cell activation previously noted in NZB mice, the surface immunoglobulin (sIg) isotypes of spleen cells from NZB mice were examined. After lactoperoxidase-catalyzed radioiodination, the cell surface immunoglobulins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Spleen cells from 8- to 10-week-old NZB mice were found to have an increased ratio of cell surface IgM/IgD compared to cells from 11 control strains. The altered ratio of sIg isotypes was not a consequence of increased proteolytic activity present in NZB cell suspensions or of the presence of cytophilic antibody or autoantibody.

Ontogenetic studies of the sIgM/sIgD (µ/) ratio on splenocytes from NZB and BALB/c mice revealed that the former cells had higher µ/ ratios as early as 2 weeks after birth. By 4 weeks of age the µ/ ratios were equivalent. Between 4 weeks and 1 year of age, the µ/ ratios on NZB splenocytes remained constant whereas those on BALB/c splenocytes decreased and reached adult levels at 6 weeks.

Footnotes

¹ This work was supported by National Institutes of Health Grants AI-11851, AI-12789, and AM-18505.

² Recipient of an Arthritis Foundation Postdoctoral Fellowship and a Clinical Investigator Award NIAMDD 1K08AM00378.

³ Recipient of a Research Career Award from the United States Public Health Service.

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