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Antigen-Induced Increase in Migration of Large Cortical Thymocytes (Regulatory Cells?) to the Marginal Zone and Red Pulp of the Spleen^1,2,

From the Departments of Pathology, State University of New York, Downstate Medical Center, Brooklyn, New York 11203, and Yale Medical School, New Haven, Connecticut 06510

Abstract

Antigen-dependent and antigen-independent thymocyte migrations are described. Rats injected i.v. with a large dose of ovalbumin (100 mg) showed a 3-fold increase in the numbers of large, low density cells in thymus 2 days later. When subpopulations of different density were labeled with ³H-adenosine and transferred to normal recipients, they migrated preferentially to spleen, unlike cells from saline-injected donors, which equilibrated among all lymphoid organs. Data from autoradiographs and dissection of spleens showed that the increased radioactivity in spleen resulted from a 3-fold increase in the number of large cells, predominantly in the marginal zone and, secondarily, in the red pulp. This increased migration to marginal zone with antigen was superimposed upon a normal pathway taken by large, low density thymocytes to this site. When adrenalectomized rats, similarly treated, received intrathymic ¹²⁵IUdR, radioactivity in spleens of the ovalbumin group after 24 hr was 2- to 3-fold greater than controls and, again, associated only with marginal zone-red pulp. When only cortical thymocytes were labeled by topical application of ¹²⁵IUdR to the thymus capsule, the same enhanced localization in spleen was seen at 24 hr. Migration of small, dense, medullary thymocytes to the periarteriolar lymphoid sheath (classical thymus-dependent area) was unaffected by antigen.

Only large, low density thymocytes migrated to Peyer's patches. This migration was unaffected by antigen. Only dense thymocytes localized in the thymus. However, if thymocytes were transferred from antigentreated donors, no subpopulation homed to thymus.

Footnotes

¹ This work was supported by American Cancer Society Grant IM-145, National Institutes of Health Grants AI-16112 and AI-06455, and National Cancer Institute Contract CB-43926.

² Reprints will not be available.