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Studies on the Immune Response to Fixed Antigens

III. Induction of Helper Function for Antibody-Dependent Cellular Cytotoxicity Responses¹

From the Lautenberg Center for General and Tumor Immunology, Hebrew University, Hadassah Medical School, Jerusalem, Israel

Abstract

Heavy trinitrophenylated sheep red cells (TNP ₁₂₈SRC) and glutaraldehyde-treated SRC (G-SRC) could not induce cellular cytotoxicity against ⁵¹Cr-SRC. In contrast, the native antigen SRC could stimulate a cytolytic response against the radiolabeled homologous target cell. However, fixed SRC could stimulate a priming function that accelerated and augmented the secondary cytotoxic response to SRC. Such fixed antigens could stimulate a delayed-type hypersensitivity (DTHS) response also. Thus, the immunologic memory to the chemically modified antigen, as well as the DTHS response, are completely dissociated from the primary cytotoxic responses.

The primary and the secondary cytotoxic responses that were developed in the spleens of the injected mice were mediated by antibody-dependent cellular cytotoxicity (ADCC), since the active supernatant that was released from the spleen cells could lyse the target cells in the presence of normal splenocytes. The active supernatant was identified as antibody. We suggest that B effector cells cytolyzed the antibody-coated target cells. Normal cells from nude mice could mediate the cytolytic process as efficiently as spleen cells from other strains of mouse. The results are discussed in terms of selective stimulation of T cell subpopulations.

Footnotes

¹ This work was supported by research grants from Concern Foundation Inc. and the Lautenberg Endowment fund. This work is part of B. Leshem's Ph.D. thesis, which was funded by Rabbi Shai Shacknai Endowment Fund.