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The Journal of Immunology, 1978, 120: 1465-1472.
Copyright © 1978 by The American Association of Immunologists, Inc.
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Immunoglobulin Secretion by Human Splenic Lymphocytes in Vitro: The Effects of Antibodies to IgM and IgD1

Fred D. Finkelman2 and Peter E. Lipsky2

From the Rheumatic Diseases Unit, Department of Internal Medicine, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Abstract

The effects of F(ab')2 fragments of affinity-purified rabbit anti-human µ chain antibody (RaHµ) and rabbit anti-human {delta} chain antibody (RaH{delta}) on spontaneous and mitogen-stimulated immunoglobulin (Ig) secretion by normal human spleen cells were studied. IgM and IgG secretion by human spleen cells cultured in vitro was measured by incubating the cells with 3H-amino acids, precipitating the secreted labeled Ig with anti-Ig, and analyzing the precipitates by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Both RaHµ and RaH{delta} suppressed spontaneous and LPS-induced IgM and IgG secretion as well as PWM-stimulated IgG secretion. In different experiments, RaHµ and RaH{delta} either suppressed or augmented PWM-induced IgM secretion. The anti-Ig induced augmentation of PWM-triggered IgM secretion was most apparent when spleen cells were cultured at lower cell densities or when lower concentrations of anti-Ig were employed. These data indicate that perturbation of B cell surface immunoglobulin receptors with specific anti-Ig antibody can alter markedly the ability of these cells to differentiate into antibody-secreting cells.

Footnotes

1 This work was supported in part by United States Public Health Service Training Grant No. AM-05154 and United States Public Health Service Research Grant No. AM-18505.

2 Recipient of an Arthritis Foundation Postdoctoral Fellowship.






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