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The Journal of Immunology, 1978, 120: 806-811.
Copyright © 1978 by The American Association of Immunologists, Inc.
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Complement-Dependent Stimulation of Normal Lymphocytes by Immune Complexes1

Lee S. F. Soderberg2,2, and Albert H. Coons

From the Department of Pathology, Harvard Medical School, 25 Shattuck Street, Boston, Massachusetts 02115

Abstract

Normal rabbit lymphocytes were stimulated to proliferate in vitro by antibody-antigen complexes. Stimulation was dependent upon C activity. Heat-inactivation or zymosan-treatment of the serum used in culture caused a 75 to 100% loss of responsiveness to the complexes. Serum-free culture or cultures with less than 1% serum supported only low levels of stimulation, but responsiveness reappeared proportionally with increased serum concentration. The low level dose-dependent responses seen in the absence of active C may have been due to C carried over with the cells or to stimulation independent of C. Aggregated rabbit {gamma}-globulin tested over a broad dose range failed to stimulate normal lymphocytes more than minimally whether or not C was present. Stimulation with immune complexes was sustained by C4-deficient guinea pig serum, indicating participation of the alternative C pathway. Normal rabbit lymphocytes from peripheral blood, bone marrow, spleen, and lymph node proliferated in response to rabbit antibody-antigen complexes. Normal thymocytes were consistently unresponsive to the complexes.

Footnotes

1 This work was supported by the National Institutes of Health Grant CA 20811.

2 L.S. was supported by the United States Public Health Service Grant AI 05342-01.

3 L.S. present address: Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Ark. 72201.






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